Induction of Coleoptile Elongation by Carbon Dioxide

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Induction of Coleoptile Elongation by Carbon Dioxide 1 Received for publication

The ability of C02 to induce elongation of Avena sativa coleoptile segments was examined with the use of a high resolution growth-recording device. CO-,saturated water causes an 8to 16-fold promotion in the rate of elongation within 1 minute. This elongation is insensitive to a variety of metabolic inhibitors that suppress auxin-induced elongation, and the C02 effect cannot be prevented by pret...

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Inhibition of cell elongation in Avena coleoptile by hydroxyproline.

A study has been made of the hydroxyproline-induced inhibition of elongation of Avena coleoptile tissues. The isomers of 4-hydroxyproline differ in their effectiveness; only the L isomers are growth inhibitors with the cis form (allohydroxyproline) being more effective than the trans form (hydroxyproline). Hydroxyproline differs from other amino acid antagonists and protein synthesis inhibitors...

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Improved Avena Coleoptile Elongation Test for Auxin'

Sum rv01,1f. A mo(lifie(l )rocedlure is presente(d for the bioassay of aulxin lusilng -Iv'eno coleoptile segments. The modifications introduicedl restult in a substantial improvement of the commonly uised( coleoptile elongatioil tests. The proposed test retains the simplicity of physical reqillremeints and of manipulatioIn characteristic of all elongatioln tests. The maiin featuire of the propo...

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Induction of euthanasia using carbon dioxide in rat: an overview of the available practical guidelines

Background and aims: Euthanasia is used to define ending an animal's life in a way that results in rapid anesthesia and death without pain or distress. One of the most common methods of performing euthanasia in rats is the administration of carbon dioxide (CO2). The aim of this study was to review the available practical guidelines for inducing euthanasia in rats by administrating CO2. M...

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Calorimetric studies of the elongation of Avena coleoptile segments.

Elongation rate and heat produced by Avena coleoptile segments suspended in sucrose buffer solutions were measured at pH values from 3.5 to 8.5. The caloric efficiency of elongation (CEE) was defined as the ratio of the rate of elongation to the rate of heat production. Elongation and CEE were greatest at intermediate pH values, but heat production (about 1 cal/g.hr) was insensitive to pH withi...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1971

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.47.3.335